]> Note on use of ND evidence code:Unlike the other evidence codes, the ND code does not indicate a method from a specificreference. Rather, it indicates that the annotator looked at the available information anddetermined that nothing is known about the gene product for a given aspect of GO(molecular function, biological process or cellular component). The annotator will alwayslook at all available literature for the gene. Depending on the resources and the annotationphilosophy of the annotating group, the annotator may also look at sequence comparisondata to determine if any predictions may be made based on the sequence. Curator reviewed annotations Annotations NOT reviewed by a curator Is annotation based on anauthor statement thatcites a published referenceas the source of information? yes NAS Is annotation based on anauthor statement that does notcite a published referenceas the source of information? yes TAS no Author statementfrom publication Is there a GO annotation in anotheraspect that allows you to make aninference based on that GO term foran aspect without evidence? yes IC no ND Have you been able to find anyevidence to support a GOannotation in a given GO aspect?[see note on use of ND] no No evidence is available Computational method Will each annotation be individuallyreviewed and confirmed by ahuman annotator? no IEA yes Is the computation an integratedanalysis, typically includingexperimental data sets, and oftenincluding multiple data types? yes ICA Does the computation includeconsideration of the genomiccontext of the gene? yes IGC no Is the computation based purely onthe sequence of the gene product(or sequence-based mapping files)? yes ISS no no yes yes yes yes yes IMP IGI IPI IDA IEP Is annotation based on adirect 1 to 1 physical interactionwith another gene product? Is annotation based on a directassay for the function, process,or component of the gene product? Is annotation based onthe expression patternof the gene product? Is more than one genebeing mutated inthe same strain? Is a single gene beingmutated or compared to otheralleles of the same gene? Is annotation based on geneticmutations or allelic variation? no no no yes Experimental(wet lab) What type of evidence is the annotation based on? GO Evidence Code Decision Tree