AmiGO Help: BLAST Search Results
Query Summary
The Query Summary displays the BLAST parameters used for the BLAST job. If more than one sequence was included in the query set, each sequence will return a separate results page. Results for each of the sequences can be viewed by clicking on the page numbers at the top of the page, or all the results can be viewed on a single page by clicking on 'View All Results'.
Query Sequence
The Query Sequence section of the BLAST results page displays the sequence used as the query sequence is displayed.
High Scoring Gene Products
The High Scoring Gene Products lists the gene products that meet the E value selected as a BLAST parameter from the query page. The results are listed in ascending order of P-values. P-value is the probability that the score was the result of chance, while E-value is the expectation value. P-value can be converted to E-value and vice versa using the following formula:
P = 1 - e-E
When E < 0.01, P-value and E-value are nearly identical.
In the column titled 'Name', gene products that show high similarity to the query sequence are listed. The name of the gene product is a hyperlink to the gene product details page for that gene or gene product.
In the column titled 'Species', the species the gene product is from is listed. The species name is a hyperlink to the NCBI Taxonomy Browser.
The checkbox on the left of each row of the table can be used to download the FASTA sequence for one or more proteins, or to get an overview of the annotations to a set of gene products. Select the gene products you are interested in, pick the appropriate option using the radio buttons at the bottom of the page, and hit Submit Query to view the results.
Raw BLAST Data
The raw BLAST results or the alignment details are displayed below the table of High Scoring Gene Products. Further details of interpreting the BLAST results are available in the NCBI BLAST Help Manual [external website].
