AmiGO Help: BLAST Query
What does the BLAST search do?
The AmiGO BLAST server searches the sequences from the GO protein sequence database, which comprises protein sequences of genes and gene products that have been annotated to a GO term and submitted to the GO Consortium. Protein queries are searched using BLASTP, while nucleotide sequences are searched using BLASTX. There is no need to specify which program to use, but if more than one sequence is submitted in single query, all sequences must be of the same type.
Entering sequences
The BLAST query form accepts three methods for submitting a query sequence:
- Enter a UniProt accession ID, for example P55269.
- Upload a file containing sequences in FASTA format. Sequences should be separated by a line break.
- Paste FASTA sequence(s) into the textbox. Sequences should be separated by a line break.
When entering more than one sequence, separate queries with a line break.
GOst allows BLAST queries of up to 100 sequences; the total number of residues cannot exceed 3 million. More information on FASTA format can be found at Wikipedia [external website].
