For more detail on all the evidence codes, see the comprehensive Evidence Code Guide.

• IEA - inferred from electronic annotation
• IC - inferred by curator
• IDA - inferred from direct assay
  • Enzyme assays
  • In vitro reconstitution (e.g. transcription)
  • Immunofluorescence (for cellular component)
  • Cell fractionation (for cellular component)
  • Physical interaction/binding
• IEP - inferred from expression pattern
  • Transcript levels (e.g. Northerns, microarray data)
  • Protein levels (e.g. Western blots)
• IGC - inferred from genomic context
  • Operon structure
  • Syntenic regions
  • Pathway analysis
  • Genome-scale analysis of processes
• IGI - inferred from genetic interaction
  • "Traditional" genetic interactions such as suppressors, synthetic lethals, etc.
  • Functional complementation
  • Rescue experiments
  • Inference about one gene drawn from the phenotype of a mutation in a different gene.
• IMP - inferred from mutant phenotype
  • Any gene mutation/knockout
  • Overexpression/ectopic expression of wild-type or mutant genes
  • Anti-sense experiments
  • RNAi experiments
  • Specific protein inhibitors
  • Polymorphism or allelic variation
• IPI - inferred from physical interaction
  • 2-hybrid interactions
  • Co-purification
  • Co-immunoprecipitation
  • Ion/protein binding experiments
• ISS - inferred from sequence or structural similarity
  • Sequence similarity (homologue of/most closely related to)
  • Recognized domains
  • Structural similarity
  • Southern blotting
• RCA - inferred from reviewed computational analysis
  • Large-scale protein-protein interaction experiments
  • Microarray experiments
  • Integration of large-scale datasets of several types
  • Text-based computation
• NAS - non-traceable author statement
• ND - no biological data available
• TAS - traceable author statement
• NR - not recorded

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